Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation

The Kluyveromyces marxianus yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order intensify the biosynthesis rate of a target product, reaching high biomass concentrations reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how achieve cell densities. However, depending on physiology particular microbial strain, optimal media composition should be used avoid by-product synthesis and, subsequently, decrease overall process effi-ciency. Thus, aim present study was optimise synthetic growth feeding solution compositions (in terms carbon, nitrogen, phosphorous, magnesium, calcium concentrations) for density K. fed?batch cultivations. Additionally, yields from vitamin mixture other macro/microelements were identified. A model predictive control algorithm successfully applied fed-batch cultivation control. Biomass substrate consumption kinetics compared with mathematical predictions. Finally, 2?phenylethanol induced productivity estimated. determined macronutrient ratio identified as C:N:P = 1:0.07:0.011. maximal attained concentration close 70 g·L-1 2-PE 0.372 g·L?1·h?1, yield 74% 2-phenylalanine.